Acylation of endogenous myelin proteolipid protein with different acyl-CoAs.
نویسندگان
چکیده
منابع مشابه
In Vivo Acylation of Rat Brain Myelin Proteolipid Protein*
Examination of brain myelin proteins by sodium dodecyl sulfate-gel electrophoresis followed by fluorography clearly showed that both proteolipid protein (PLP) and DM-20 were acylated 24 h after the intracerebral injection of 30-day-old rats with C3H]palmitic acid. The radioactivity associated with PLP remained after purification, re-electrophoresis, and fluorography. Most of the radioactivity a...
متن کاملCell-free acylation of rat brain myelin proteolipid protein and DM-20.
Incubation of rat brain myelin with [3H]palmitic acid in the presence of ATP, CoA and MgCl2 or [14C]-palmitoyl-CoA in a cell-free system resulted in the selective labelling of 'PLP' [proteolipid protein; Folch & Lees (1951) J. Biol. Chem. 191, 807-817] and 'DM-20' [Agrawal, Burton, Fishman, Mitchell & Prensky (1972) J. Neurochem. 19, 2083-2089] which, after polyacrylamide-gel electrophoresis in...
متن کاملassociation of new putative epitopes of myelin proteolipid protein (58-74) with pathogenesis of multiple sclerosis
multiple sclerosis (ms) is an autoimmune disease in which auto-reactive t cells react with self-antigens expressed in the central nervous system (cns). the main cause of ms is unknown. nonetheless, the most probable theory is based on molecular mimicry, which suggests that some infections can activate t cells against brain auto-antigens like myelin proteolipid protein (plp) and initiate the dis...
متن کاملMyelin basic protein is affected by reduced synthesis of myelin proteolipid protein in the jimpy mouse.
Myelin basic proteins (MBPs) from 6-day-old, 10-day-old, 20-day-old and adult normal mouse brain were compared with those from 20-day-old jimpy (dysmyelinating mutant) mouse brain to determine the effect of reduced levels of proteolipid protein (PLP) on MBPs. Alkaline-urea-gel electrophoresis showed that 6-day-old and 10-day-old normal and jimpy MBPs lacked charge microheterogeneity, since C8 (...
متن کاملAssembly of CNS Myelin in the Absence of Proteolipid Protein
Two proteolipid proteins, PLP and DM20, are the major membrane components of central nervous system (CNS) myelin. Mutations of the X-linked PLP/DM20 gene cause dysmyelination in mouse and man and result in significant mortality. Here we show that mutant mice that lack expression of a targeted PLP gene fail to exhibit the known dysmyelinated phenotype. Unable to encode PLP/DM20 or PLP-related po...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1987
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)61628-x